Reverse-phase high-performance liquid chromatographic assay of phospholipases: application of spectrophotometric detection to rat phospholipase A2 isozymes

This paper appraises an HPLC method for assaying phospholipase A2 (PLA2).The procedure is based on heptane-isopropanol-H2SO4 extraction of fatty acids released by the enzyme in the presence of margaric acid as an internal standard, and precolumn derivatization with 9-anthryldiazomethane.The derivatives of naturally occurring long-chain fatty acids

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Synthesis and Thermal Properties of Novel Biodegradable ABCBA Pentablock Copolymers from Poly (Ethylene glycol), L-Lactide and p-Dioxanone

In this work, new biodegradable ABCBA type pentablock copolymers with different mole wig ratio of L-lactide and PPDO-b-PEG-b-PPDO triblock copolymer were synthesized and characterized.In the first step, PPDO-b-PEG-b-PPDO triblock copolymer was synthesized via a ring-opening polymerization of P-DiOxanone (PDO) monomer with Poly (Ethylene Glycol) (PE

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Deubiquitination of epidermal growth factor receptor by ubiquitin-specific peptidase 54 enhances drug sensitivity to gefitinib in gefitinib-resistant non-small cell lung cancer cells.

Youth Roping Style Saddle A precise balance between ubiquitination and deubiquitination is crucial for cellular regulation.Ubiquitin-specific peptidase 54 (USP54), an active deubiquitinase (DUB), modulates the ubiquitination of the epidermal growth factor receptor (EGFR).While the significance of USP54 in tumorigenesis is known, its specific functi

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